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Biological circuit engineers will need to develop methods to account for the disparate and often heavy physiological burdens that synthetic systems place on their microbial hosts, perhaps borrowing lessons from metabolic engineering, in which _______________________________ has been used to overcome similar hurdles.

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Synthetic circuit design will (and should) increasingly place emphasis on the ability to use circuits for practical applications. In the context of metabolic engineering, the ability of engineered circuits to interact with cellular systems to dynamically ______________________________ is one near-term challenge.

In the near future, workflow for a biological circuit engineer will no longer be limited by the pace of fabrication but instead by their ability to analyze ______________________ and incorporate the data into the next design cycle.

The awareness of various ethical and safetyissues by the public, industrials and governments and the readiness to participate in a dialogue provide a positive _________________ for a fruitful ethical assessment of artificial life and synthetic biology.

The thought that human beings might soon be capable of synthesizing and controlling life evokes scenarios and _________ which are particularly concrete precisely because the idea of artificial life designed by human beings is not new but a recurrent topic in literature, film, and philosophy.

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