题目内容

To avoid ligation of separate DNA fragments, which of the enzyme is used? ____________

A.phosphatase
B.kinase
C.ligase
D.endonuclease

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The loop region is single stranded. It can be cleaved by using which enzyme? ____________

A.Exonuclease
B.S1 nuclease
C.RNaseH
D.DNase

Which of the following refers to the inactivation of Insertional inactivation? ___________

A.Host
B.Gene of Interest
C.Gene of the vector
D.Gene of the host

BamHI cuts the pBR322 at just one position, within the cluster of genes that code for resistance to ___________

A.Tetracycline
B.Ampicillin
C.Kanamycin
D.Gentamycin

On a medium containing ampicillin, when pBR322 recombinants were plated. Which of the following will not be able to grow? ___________

A. pBR322 containing gene of interest
B.self-ligated Pbr22
C.non-transformants
D.transformed cells