sum([i*i for i in range(3)])的计算结果是( )。
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A reaction mixture for PCR consists of ____________
A.heat unstable polymerase
B.primers in a limited amount
C.deoxynucleoside triphosphate (dNTPs)
D.a region complementary to the sequence to be amplified
These are steps taken in carrying out the PCR reaction: i) Attaching of primers by cooling ii) Denaturation of strands iii) DNA synthesis iv) Heating Which is the correct order? (Mentioned from starting to ending the reaction)
A.i)-ii)-iii)-iv)
B.ii)-i)-iii)-iv)
C.iv)-iii)-ii)-i)
D.iv)-ii)-i)-iii)
Choose the correct statement.
A. Taq polymerase is having very high processivity
B. Processivity is defined in this case as a synthesis of DNA by polymerase
C. It requires a 5’ end for the elongation to take place
D. The maximum size of the molecules which can be synthesized is 10kbp
Which of the following activity is not present in Taq polymerase?
A. 5’-3’ polymerase
B. 5’-3’ exonuclease
C. 3’-5’ exonuclease
D. Both 5’-3’ polymerase and 5’-3’ exonuclease
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