An alternative approach to determining protein–protein interactions is to use a large-scale affinity purification technique that involves attaching fusion tags to proteins and purifying the associated protein complexes in an affinity chromatography column.
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Which of the following is an incorrect statement?
A.SAGE and DNA microarrays are both high throughput techniques that determine global mRNA expression levels
B.Studies have indicated that the gene expression measurements from these methods are highly inconsistent with each other
C.SAGE does not require prior knowledge of the transcript sequence
D.DNA microarray experiments can only detect the genes spotted on the microarray
DNA microarrays measure “absolute” mRNA expression levels without arbitrary reference standards, whereas SAGE indicates the relative expression levels.
A.True
B.False
Which of the following is incorrect regarding 2D-Page?
A.It stands for Two-dimensional polyacrylamide gel electrophoresis
B.It separates proteins by charge only
C.The gel is run in one direction in a pH gradient under a non-denaturing condition
D.It works to separate proteins by isoelectric points (pI)
理想运算放大器的两个重要结论是（ ）。
A. 虚地与反相
B. 虚短与虚地
C. 虚短与虚断
D. 短路与断路
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