题目内容

Which of the following is correct with respect to caesium chloride centrifugation()

A. Caesium is light in weight
B. The dissolution of caesium and nucleic acids leads to the formation of gradients
C. According to the amount of supercoiling and A+T content, the DNA settles
D. Nicked DNA settles below than supercoiled DNA

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Please select the true statement(s) using vectors to make recombinant DNA()

A. The DNA of interest could not replicate if it were not in a plasmid
B. The vector prevents degradation of DNA by phages
C. Vectors are more well-characterized than chromosomal DNA
D. They accept longer DNA fragments

Chromosomal or genomic DNA is separated by______()

A. Sedimentation
B. Dissolution in water
Centrifugation
Distillation

Which of the following is used to lyse the nucleus and release the DNA()

A. sodium dodecyl sulfate (SDS)
B. ammonium sulfate
C. ferric phosphate
D. phenol

What is(are) the feature(s) associated with M13 phage()

A. Doesn’t need genes for insertion into host genome
B. Capsid constructed of multiple copies of 3 genes
C. Simpler infection cycle than lambda
D. Capsid constructed of 15 different proteins

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