题目内容

What are cosmid vectors（）

A. Hybrid between phage and plasmid
B. Hybrid between M13 and lambda phage
C. Modified lambda vector
D. Modified M13 vector

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Which is an example of a simplest vector （in terms of size）（）

A. 2 micron circle
Bacteriophage
C. Plasmid
D. YAC

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The first step in a PCR cycle is to heat the sample to 95°C. What is the purpose of heating the sample（）

A. To allow the primer to anneal to the template
B. To separate the two strands of DNA
C. To produce the optimal temperature for Taq dna polymerase to work
D. To produce the optimal temperature for most enzymatic reactions

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The first step in replication is separating the two strands of DNA. This task is achieved by the enzyme helicase, which unwinds the double stranded DNA. What does this create（）

A replication fork
B. A transcription bubble
C. A DNA Zipper
D. Primase

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During replication, which enzyme is responsible for making a daughter strand of DNA using the parent strand as template（）

A. Reverse transcriptase
B. RNAse
C. DNA polymerase
D. RNA polymerase

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What are cosmid vectors（）

A. Hybrid between phage and plasmid B. Hybrid between M13 and lambda phage C. Modified lambda vector D. Modified M13 vector

Which is an example of a simplest vector （in terms of size）（）

The first step in a PCR cycle is to heat the sample to 95°C. What is the purpose of heating the sample（）

The first step in replication is separating the two strands of DNA. This task is achieved by the enzyme helicase, which unwinds the double stranded DNA. What does this create（）

During replication, which enzyme is responsible for making a daughter strand of DNA using the parent strand as template（）

A. Hybrid between phage and plasmid
B. Hybrid between M13 and lambda phage
C. Modified lambda vector
D. Modified M13 vector