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In June 2019, Olsman and Paulsson report an analogous system for chemical reactions in living cells. Specifically, they design a reaction module in which two components sequester each other, and show that adding this to almost any network can force the output of the system to maintain a precise value that is proportional to an input signal, in a way that is robust to both external disturbances and uncertainty in the internal parameters — a behavior known as ______________________________.

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Synthetic biology itself can provide new effective biosecurity mechanisms, such as synthetic auxotrophies, ______________________ or ingenious genetic circuits.

Biological circuit engineers will need to develop methods to account for the disparate and often heavy physiological burdens that synthetic systems place on their microbial hosts, perhaps borrowing lessons from metabolic engineering, in which _______________________________ has been used to overcome similar hurdles.

Synthetic circuit design will (and should) increasingly place emphasis on the ability to use circuits for practical applications. In the context of metabolic engineering, the ability of engineered circuits to interact with cellular systems to dynamically ______________________________ is one near-term challenge.

In the near future, workflow for a biological circuit engineer will no longer be limited by the pace of fabrication but instead by their ability to analyze ______________________ and incorporate the data into the next design cycle.