What is an advantage of using a bacteriophage, compared with a plasmid()
A. Phages can be used to kill bacteria, and sterilize an area
B. Plasmids do not contain restriction sites
C. Bacteriophages can carry much longer DNA fragments than plasmids
D. Bacteriophages can carry smaller fragments than plasmids
E. Plasmids will not reproduce within bacteria
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Once the recombinant plasmid is taken up by a host (E.coli), what must a researcher do()
A. Insert the DNA into a phage
B. Raise the host cells and select colonies containing the plasmid
C. Use one or more restriction enzymes
D. Ligate the DNA fragment to the plasmid
What are cosmid vectors()
A. Hybrid between phage and plasmid
B. Hybrid between M13 and lambda phage
C. Modified lambda vector
D. Modified M13 vector
Which is an example of a simplest vector (in terms of size)()
A. 2 micron circle
Bacteriophage
C. Plasmid
D. YAC
The first step in a PCR cycle is to heat the sample to 95°C. What is the purpose of heating the sample()
A. To allow the primer to anneal to the template
B. To separate the two strands of DNA
C. To produce the optimal temperature for Taq dna polymerase to work
D. To produce the optimal temperature for most enzymatic reactions