Which of the following statements about the definition of 'gene therapy' byFarlex Partner Medical Dictionary are correct?
A. Gene therapy is alteration of somatic or germ-line DNA to correct or prevent disease; the process of inserting a gene artificially into the genome of organism to genetic defect or to add a new biologic property or function with therapeutic potential.
B. In somatic gene therapy, functional DNA sequences are inserted into cells that lack a specific gene or bear a faulty version of it. Vectors include replication-defective viruses, liposomes, and plasmids.
C. For transfer of genetic material by viral infection (called transduction), retroviruses are particularly suitable as vectors because their RNA, converted to DNA by reverse transcriptase, becomes part of the genome of the infected cell. Adenovirus and herpesvirus are also used.
D. Progress has been made in treating several inherited disorders, including severe combined immunodeficiency, cystic fibrosis, and hemophilia B.
查看答案
Whichofthefollowingstatementsaboutthedefinitionof'genetherapy'byFarlexPartnerMedicalDictionaryarecorrect?
A. Gene therapy has several applications in oncology, including the transduction into malignant tumor cells of genes encoding cytokines or coactivation factors to augment host antitumor responses and the transfer of tumor suppressor genes, particularly p53 (the most commonly mutated gene found in human cancers), to enhance the sensitivity of malignant cells to chemotherapeutic agents.
B. Use of viral vectors is associated with a risk of localized and systemic inflammation mediated by cytokines, which can be fatal.
C. Germ-line therapy inserts specific genes directly into the DNA of sperm, egg, or embryo, producing heritable alterations of the genome.
D. Progresshasbeenmadeintreatingseveralinheriteddisorders,includingseverecombinedimmunodeficiency,cysticfibrosis,andhemophiliaB.
Which of the following statements about viral vectors for gene therapy are correct?
A. In order to replicate, viruses introduce their genetic material into the host cell, tricking the host’s cellular machinery into using it as blueprints for viral proteins.
B. Retroviruses go a stage further by having their genetic material copied into the genome of the host cell.
C. A number of viruses have been used for human gene therapy, including retroviruses, adenoviruses, herpes simplex, vaccinia, and adeno-associated virus (AAV).
D. Like the genetic material (DNA or RNA) in viruses, therapeutic DNA can be designed to simply serve as a temporary blueprint that is degraded naturally or (at least theoretically) to enter the host's genome, becoming a permanent part of the host's DNA in infected cells.
Whichofthefollowingstatementsabout non-viralvectorsforgenetherapyarecorrect?
A. Non-viral methods present certain advantages over viral methods, such as large scale production and low host immunogenicity.
B. However, non-viral methods initially produced lower levels of transfection and gene expression, and thus lower therapeutic efficacy.
C. Later technology remedied this deficiency.
D. Methods for non-viral gene therapy include the injection of naked DNA, electroporation, the gene gun, sonoporation, magnetofection, the use of oligonucleotides, lipoplexes, dendrimers, and inorganic nanoparticles.
Which of the following viruses are commonly used as vectors in gene therapy?
A. adenovirus
B. AAV
C. retrovirus
D. vaccinia virus
微信支付 
支付宝支付