简答题

    Part II Please translate the two paragraphs into Chinese (Total Score:40).1. Monoclonal antibodies(单克隆抗体) have a wide range of uses as very precise and sensitive analytical reagents for various pharmaceutical products such as peptides, proteins and hormones. They are also important as diagnostic reagents for a number of medical and therapeutic purposes, including screening for the presence of undesirable agents in blood samples used for transfusions. A combination of genetic manipulation and monoclonal antibody techniques might well give rise to new approaches to efficient production of viral, bacterial and parasitic antibodies. Other important uses will include tissue typing, in vivo tumour location, clinical diagnosis and therapy including targeting of chemotherapeutic agents.2. We have identified a novel means to achieve substantially increased vegetative biomass and oilseed production in the model plant Arabidopsis thaliana. Endogenous isoforms of starch branching enzyme (SBE) were substituted by either one of the endosperm expressed maize (Zea mays L.) branching isozymes, ZmSBEI or ZmSBEIIb. Transformants were compared with the starch-free background and with the wild-type plants. Each of the maize-derived SBEs restored starch biosynthesis but both morphology and structure of starch particles were altered. Altered starch metabolism in the transformants is associated with enhanced biomass formation and more-than-trebled oilseed production while maintaining seed oil quality. Enhanced oilseed production is primarily due to an increased number of siliques(角果) per plant whereas oil content and seed number per silique are essentially unchanged or even modestly decreased. Introduction of cereal starch branching isozymes into oilseed plants represents a potentially useful strategy to increase biomass and oilseed production in related crops and manipulate the structure and properties of leaf starch.


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