请你提供两份计划:第一份要确证只有单一序列DNA被测序,第二份应该保证只有被测序的单一序列DNA是可转录的。
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On the basis of this passage, what may be concluded about the real origin of stories “Sleeping Beauty” and “Cinderella”?
A. They were invented by Elizabeth Vergoose.
B. They were invented by Thomas Fleet.
C. They were invented by Charles Perrault.
D. Their writers are unknown.
概述机体对某特定抗原产生大量抗体并一直维持这种能力的机制。
The judge gave each man a stick _______.
A. to change back the farmer’s corn
B. to beat the thief
C. as a tool to find out the thief
D. so as to play a game together
真核细胞编码蛋白质基因的启动子包含了一个基本的启动元(promotor element),这个启动元可被RNA聚合酶Ⅱ以及一些基本的转录因子(如TFⅡD,TFⅡB等)识别。但是启动子自身的基本活性很低,而且结合于启动子邻近上游区(-120~-30)或更远的增强小区域的其他特殊转录因子,对它有不变的影响。分析这些调控区发现它们一般包含有许多不同的序列特异性转录因子的结合位点。
用转染试验分析这样一个复合物调控区的某一单因子识别位点表明,结合位点的活性较低。但是连接这一结合位点的多重拷贝在一起经常会产生协同促进作用。下面显示的是关于转录因子FE结合位点的DNA保守序列(20bp)的数据。克隆这个保守DNA序列在基础启动子(basal promoter)和报道基因(reporter gene)的上游。并导入细胞培养48hr。如何叙述该实验中的激活模式?