题目内容

The type of DNA amplification where the region of DNA amplified lies on either side of a known segment __________

A.RT – PCR
B.Anchored – PCR
C.Inverse – PCR
D.Nested – PCR

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Why internal secondary structures are not preferred for primers? __________

A.Internal structures are very bulky and thus elongation is not preferred
B.Because of it, primer may fold back on itself and won’t be available for template
C.Internal secondary structures require more amount of template
D.If internal structures are present, no proof reading would be observed

Which of the following nucleotides should be there at 3’ end?

A.Any of A, T, G or C will work out
B.Either A or T
C.Either G or C
D.Specifically G

刚性车钩

从在列车编组中的位置以及连挂、解钩作业两方面分析全自动车钩、半自动车钩与半永久牵引杆的区别。

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