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Due to the heat stability of bacterial spores, which cannot be removed by boiling, sterilization using moist heat must be done at highest temperature and pressure in an autoclave.
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These machines normally operate at 121℃ and 15 pounds per square inch which is sufficient to kill most microbes with the exception of spongiform encephalopathic agents, which are resistant to these conditions.
The length of time used for sterilisation depends on the volume of the materials.
Dry heat is routinely used in the laboratory to sterilize wire loops and remove microhes from the rims of culture vessels by flaming in a bunsen flame.
Heat-labile materials can be sterilised by filtration or by radiation.
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