Which of the following is untrue regarding the classic yeast two-hybrid method?
A.It is used for the detection of Protein interactions
B.Method that relies on the interaction of “bait” and “prey” proteins in molecular constructs in yeast
C.DNA-binding domain and a trans-activation domain don’t necessarily interact
D.In this strategy, a two-domain transcriptional activator is employed as a helper for determining protein–protein interactions
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An alternative approach to determining protein–protein interactions is to use a large-scale affinity purification technique that involves attaching fusion tags to proteins and purifying the associated protein complexes in an affinity chromatography column.
A.True
B.False
Which of the following is an incorrect statement?
A.SAGE and DNA microarrays are both high throughput techniques that determine global mRNA expression levels
B.Studies have indicated that the gene expression measurements from these methods are highly inconsistent with each other
C.SAGE does not require prior knowledge of the transcript sequence
D.DNA microarray experiments can only detect the genes spotted on the microarray
DNA microarrays measure “absolute” mRNA expression levels without arbitrary reference standards, whereas SAGE indicates the relative expression levels.
A.True
B.False
Which of the following is incorrect regarding 2D-Page?
A.It stands for Two-dimensional polyacrylamide gel electrophoresis
B.It separates proteins by charge only
C.The gel is run in one direction in a pH gradient under a non-denaturing condition
D.It works to separate proteins by isoelectric points (pI)
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