During DNA cloning which of the following is not a crucial requirement? ____________
A. DNA inserts
B. Vector
C. Protein expression
D. Molecular cutter
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Which of the following statements is correct regarding S1 nuclease? ____________
A.It acts on double stranded DNA
B.It acts on single stranded DNA
C.It acts on both types of strands
D.It is obtained from coli
Bal31 is also an enzyme which is used. Which of the following statements hold true in its context? ____________
A. It is having only 3’-5’ exonuclease activity and no endonuclease activity
B. It is having only an endonuclease activity
C. It is having 5’-3’ exonuclease activity
D. It leads to shortening from both the ends
Enzyme commonly used for carrying out ligation reaction is ____________
A. Transferase
B.Reverse transciptase
C. Ligase
DNase
Which of the following is false about klenow fragment? ____________
A. Polymerization activity
B. 3’→5’ exonuclease activity
C. 5’→3’ exonuclease activity
D. 324 – 928 residue of polymerase I
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