题目内容

Once cosmids are inside the E.coli cells, they don’t generate phage but are propagated as plasmids.__________

A.True
B.False

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Which size of the insert is accepted by the cosmids? __________

A. 10-20 kbp
B. 35-45 kbp
C. 50-60 kbp
D.100-120 kbp

The plaques formed by M13 infection are called as __________

A.retarded plaques
B.true plaques
C.pseudoplaques
D.lysogenized plaques

The main advantage of phagemid is that it can be used for the generation of single or double stranded products without recloning. __________

A.True
B.False

Alpha complementation is an indicator of lacZ system. Which of the statement is incorrect for it? __________

A.One portion of the lacZ gene known as minigene is present in the plasmid
B.Another portion is present in the host itself
C.If they both are allowed to combine in the presence of IPTG, X-gal and ampicillin, blue colonies are observed
D.If insert is also present along with host and plasmid, it results in the formation of blue colonies

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