Which enzyme is used for the replication in case of Sanger’s method of sequencing? ____________
A.Polymerase I
B.Smaller subunit polymerase I
C.Polymerase III
D.Larger subunit polymerase I
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With respect to Sanger’s method of sequencing, pick the odd one out. ____________
A.Radioactive dideoxyribonucleotides
B.Primers
C. Klenow fragment
D.Restriction digestion
No chain termination takes place in automated sequencing ____________
A.True
B.False
Automated sequencing is a real time one trial method of sequencing and is highly handy. ____________
A.True
B.False
Which of the following is used by DNA polymerase as a substrate? ____________
A.Sucrose
B.Lactose
C.Nucleotide
D.Nucleoside