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Methods for post-translational regulation are still in their infancy, as a generalized system to control synthetic and endogenous proteins has not yet been reported. As synthetic systems have become larger and lesser complex, their interactions with endogenous systems have become more pronounced.
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The development of cell-based therapeutic strategies in which engineered microorganisms interface with the human gut microbiota to fight infection and chronic disease is a challenge.
The pace of progress in synthetic biology will continue to accelerate as design and testing cycles rely more on the traditional molecular cloning tools that sustained the field in its early years and increasingly on DNA synthesis and high-throughput assembly methods.
Artificial life and synthetic biology may raise various ethical and safety issues and these issues should be carefully assessed before these novel technologies are widely distributed and applied.
Artificial life and synthetic biology is a fascinating field not only for scientists and engineers involved in it but for anybody interested in its aims and ideas.
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