The loop region is single stranded. It can be cleaved by using which enzyme? ____________
A.Exonuclease
B.S1 nuclease
C.RNaseH
D.DNase
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Which of the following refers to the inactivation of Insertional inactivation? ___________
A.Host
B.Gene of Interest
C.Gene of the vector
D.Gene of the host
BamHI cuts the pBR322 at just one position, within the cluster of genes that code for resistance to ___________
A.Tetracycline
B.Ampicillin
C.Kanamycin
D.Gentamycin
On a medium containing ampicillin, when pBR322 recombinants were plated. Which of the following will not be able to grow? ___________
A. pBR322 containing gene of interest
B.self-ligated Pbr22
C.non-transformants
D.transformed cells
In screening for pUC8 recombinants, which color colonies will be the desired recombinants? ___________
A.Blue
B.Colorless
C.White
D.Yellowish