Expression of T7 promoter- lac operator hybrid requires ___________
A.T7 RNA polymerase
B.An inducer such as IPTG
C.Both T7 RNA polymerase and inducer such as IPTG
D.T7 DNA polymerase
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Which of the following is a wrong statement?
A.To assume biological activity, many nascent polypeptides have to be covalently modified before or after the folding process
B.In eukaryotic cells most modifications take place in the endoplasmic reticulum and the Golgi apparatus
C.The modifications in eukaryotic cells include proteolytic cleavage; formation of disulfide bonds; addition of phosphoryl, methyl, acetyl, or other groups onto certain amino acid residues
D.The modifications in eukaryotic cells doesn’t include attachment of oligosaccharides or prosthetic groups to create mature proteins
Translation starts at first _____ codon in the mRNA downstream from the ribosome binding site.
A.ACG
B.AUG
C.UAC
D.CAG
In fusion proteins, the reading frame in a vector is out of phase with the insert.
A.True
B.False
There are some advantages of expressing protein as a fusion protein. It may enhance stability, folding, ______ and ______ formation.
A.solubility, phosphodiester bond formation
B.insolubility, phosphodiester bond formation
C.solubility, disulphide bond formation
D.insolubility, disulphidebond formation
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