超星 - Unit Test 13

单选题

Lambda PL promoter is used in which vectors?

A.Cloning vectors
B.Expression vectors
C. Both cloning and expression vectors
D.Bacteriophage Mu

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单选题

tac promoter is an example of which type of promoter?

A.hybrid promoter
B.fusion promoter
C.lacZ promoter
D.araBAD promoter

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单选题

Expression of T7 promoter- lac operator hybrid requires ___________

A.T7 RNA polymerase
B.An inducer such as IPTG
C.Both T7 RNA polymerase and inducer such as IPTG
D.T7 DNA polymerase

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单选题

Which of the following is a wrong statement?

A.To assume biological activity, many nascent polypeptides have to be covalently modified before or after the folding process
B.In eukaryotic cells most modifications take place in the endoplasmic reticulum and the Golgi apparatus
C.The modifications in eukaryotic cells include proteolytic cleavage; formation of disulfide bonds; addition of phosphoryl, methyl, acetyl, or other groups onto certain amino acid residues
D.The modifications in eukaryotic cells doesn’t include attachment of oligosaccharides or prosthetic groups to create mature proteins

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单选题

Translation starts at first _____ codon in the mRNA downstream from the ribosome binding site.

A.ACG
B.AUG
C.UAC
D.CAG

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单选题

In fusion proteins, the reading frame in a vector is out of phase with the insert.

A.True
B.False

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单选题

There are some advantages of expressing protein as a fusion protein. It may enhance stability, folding, and formation.

A.solubility, phosphodiester bond formation
B.insolubility, phosphodiester bond formation
C.solubility, disulphide bond formation
D.insolubility, disulphidebond formation

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单选题

Often, protein to be expressed is fused with histidine and it is called as histidine tags. For their purification, matrix containing ______ is used.

A.calcium ions
B.nickel ions
C.iron ions
D.fluorine ions

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单选题

GFP is one of a marker which is used for screening libraries in hosts other than E. coli. Choose the incorrect statement for GFP.

A.It stands for Green Fluorescent Protein
B.It is obtained from a bio-luminescent jellyfish and produces protein aequorin which emits blue light
C.The blue light is produced because of binding of sodium ions
D.The absorbed blue light produces green light which can be detected further

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单选题

Luciferase genes are also used at times for detection. Choose the correct statement for them.

A.They are obtained from fire flies only
B.The detection requires provision of substrate which produces light
C.Enzymes such as beta-galactosidase requires substrate X-gluc to produce light
D.Lucifearse genes are preferred over fluorescent proteins

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Lambda PL promoter is used in which vectors?

tac promoter is an example of which type of promoter?

Expression of T7 promoter- lac operator hybrid requires ___________

Which of the following is a wrong statement?

Translation starts at first _____ codon in the mRNA downstream from the ribosome binding site.

In fusion proteins, the reading frame in a vector is out of phase with the insert.

There are some advantages of expressing protein as a fusion protein. It may enhance stability, folding, ______ and ______ formation.

Often, protein to be expressed is fused with histidine and it is called as histidine tags. For their purification, matrix containing ______ is used.

GFP is one of a marker which is used for screening libraries in hosts other than E. coli. Choose the incorrect statement for GFP.

Luciferase genes are also used at times for detection. Choose the correct statement for them.

There are some advantages of expressing protein as a fusion protein. It may enhance stability, folding, and formation.