In fusion proteins, the reading frame in a vector is out of phase with the insert.
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There are some advantages of expressing protein as a fusion protein. It may enhance stability, folding, ______ and ______ formation.
A.solubility, phosphodiester bond formation
B.insolubility, phosphodiester bond formation
C.solubility, disulphide bond formation
D.insolubility, disulphidebond formation
Often, protein to be expressed is fused with histidine and it is called as histidine tags. For their purification, matrix containing ______ is used.
A.calcium ions
B.nickel ions
C.iron ions
D.fluorine ions
GFP is one of a marker which is used for screening libraries in hosts other than E. coli. Choose the incorrect statement for GFP.
A.It stands for Green Fluorescent Protein
B.It is obtained from a bio-luminescent jellyfish and produces protein aequorin which emits blue light
C.The blue light is produced because of binding of sodium ions
D.The absorbed blue light produces green light which can be detected further
Luciferase genes are also used at times for detection. Choose the correct statement for them.
A.They are obtained from fire flies only
B.The detection requires provision of substrate which produces light
C.Enzymes such as beta-galactosidase requires substrate X-gluc to produce light
D.Lucifearse genes are preferred over fluorescent proteins
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