The digestion of mRNA during RT – PCR is carried out by the enzyme ____________
A.Exonuclease
B.RNase H
C.Bal 31
D.Endonuclease
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Which of the following is a mismatch? ____________
A.Polymerase – Taq polymerase
B.Template – double stranded DNA
C.Primer – oligonucleotide
D.Synthesis – 5’ to 3’ direction
At what temperature do annealing of DNA and primer takes place? ____________
A.42˚
B.54˚
C.74˚
D.96˚
The type of DNA amplification where the region of DNA amplified lies on either side of a known segment __________
A.RT – PCR
B.Anchored – PCR
C.Inverse – PCR
D.Nested – PCR
Why internal secondary structures are not preferred for primers? __________
A.Internal structures are very bulky and thus elongation is not preferred
B.Because of it, primer may fold back on itself and won’t be available for template
C.Internal secondary structures require more amount of template
D.If internal structures are present, no proof reading would be observed
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