题目内容

某种酶的粗提液，每毫升含有20mg蛋白质，在0.5ml的标准反应体系中，含有10ul的这种酶提取液，在最适条件下，在一分钟内生成30nmol产物。求（1）酶的反应速度v（nmol/L.min或umol/L.min）；（2）若用10ul的酶提取液在1ml总反应体系中进行实验，其反应的速度是多少？（3）在反应混合液和酶的提取液中酶的浓度各是多少？（4）酶制剂的比活力是多少？（浙江大学考研）

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骨骼肌粗的无细胞提取液，每毫升含有32 mg蛋白质。在最适的条件下，10μl提取液的催化反应速度为0.14μmol／min。取50 ml提取液用硫酸铵沉淀法分离。将饱和度为20％至40％的沉淀物，再溶于10 ml水中，这个溶液的蛋白质浓度为50 mg／ml。从中取出10μl测定其反应速度为0.65μmol／min。计算：(1)提纯过程中酶的回收率；(2)酶的纯化倍数。

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