These are steps taken in carrying out the PCR reaction: i) Attaching of primers by cooling ii) Denaturation of strands iii) DNA synthesis iv) Heating Which is the correct order? (Mentioned from starting to ending the reaction)
A.i)-ii)-iii)-iv)
B.ii)-i)-iii)-iv)
C.iv)-iii)-ii)-i)
D.iv)-ii)-i)-iii)
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Choose the correct statement.
A. Taq polymerase is having very high processivity
B. Processivity is defined in this case as a synthesis of DNA by polymerase
C. It requires a 5’ end for the elongation to take place
D. The maximum size of the molecules which can be synthesized is 10kbp
Which of the following activity is not present in Taq polymerase?
A. 5’-3’ polymerase
B. 5’-3’ exonuclease
C. 3’-5’ exonuclease
D. Both 5’-3’ polymerase and 5’-3’ exonuclease
Which end of the primer should be matched properly in order to carry out the amplification?
A.5’ end
B.3’ end
C.Both of the ends should be matched properly
D.Anyone of the ends should match
Melting temperature is given by ____________
A.4(G+C) + 2(A+T)
B.2(G+C) + 4(A+T)
C.2(A+G) + 4(C+T)
D.4(A+G) + 2(C+T)
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