Which of the following activity is not present in Taq polymerase?
A. 5’-3’ polymerase
B. 5’-3’ exonuclease
C. 3’-5’ exonuclease
D. Both 5’-3’ polymerase and 5’-3’ exonuclease
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Which end of the primer should be matched properly in order to carry out the amplification?
A.5’ end
B.3’ end
C.Both of the ends should be matched properly
D.Anyone of the ends should match
Melting temperature is given by ____________
A.4(G+C) + 2(A+T)
B.2(G+C) + 4(A+T)
C.2(A+G) + 4(C+T)
D.4(A+G) + 2(C+T)
What will happen if the amino acid sequence is used directly for primer designing?
A.There would be certainty because the genetic code is unique for each amino acid
B.There would be uncertainty as the genetic code is degenerate and none of the amino acid is having a unique code
C.There would be uncertainty as the genetic code is degenerate but some of the amino acids such as methionine are having a unique codon
D.The amount of uncertainty or certainty is a matter of chance
Which of the following is favored for primer design?
A.The melting temperature should be different for both the primers
B.Primers should be long in length
C.Primers should not be complementary to each other
D.Matching should be of whole primer to the template
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