Which of the following is favored for primer design?
A.The melting temperature should be different for both the primers
B.Primers should be long in length
C.Primers should not be complementary to each other
D.Matching should be of whole primer to the template
查看答案
Reverse transcriptase produces DNA from RNA _______
A.True
B.False
The digestion of mRNA during RT – PCR is carried out by the enzyme ____________
A.Exonuclease
B.RNase H
C.Bal 31
D.Endonuclease
Which of the following is a mismatch? ____________
A.Polymerase – Taq polymerase
B.Template – double stranded DNA
C.Primer – oligonucleotide
D.Synthesis – 5’ to 3’ direction
At what temperature do annealing of DNA and primer takes place? ____________
A.42˚
B.54˚
C.74˚
D.96˚
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