Reverse transcriptase produces DNA from RNA _______
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The digestion of mRNA during RT – PCR is carried out by the enzyme ____________
A.Exonuclease
B.RNase H
C.Bal 31
D.Endonuclease
Which of the following is a mismatch? ____________
A.Polymerase – Taq polymerase
B.Template – double stranded DNA
C.Primer – oligonucleotide
D.Synthesis – 5’ to 3’ direction
At what temperature do annealing of DNA and primer takes place? ____________
A.42˚
B.54˚
C.74˚
D.96˚
The type of DNA amplification where the region of DNA amplified lies on either side of a known segment __________
A.RT – PCR
B.Anchored – PCR
C.Inverse – PCR
D.Nested – PCR