What will happen if the amino acid sequence is used directly for primer designing?
A.There would be certainty because the genetic code is unique for each amino acid
B.There would be uncertainty as the genetic code is degenerate and none of the amino acid is having a unique code
C.There would be uncertainty as the genetic code is degenerate but some of the amino acids such as methionine are having a unique codon
D.The amount of uncertainty or certainty is a matter of chance
查看答案
Which of the following is favored for primer design?
A.The melting temperature should be different for both the primers
B.Primers should be long in length
C.Primers should not be complementary to each other
D.Matching should be of whole primer to the template
Reverse transcriptase produces DNA from RNA _______
A.True
B.False
The digestion of mRNA during RT – PCR is carried out by the enzyme ____________
A.Exonuclease
B.RNase H
C.Bal 31
D.Endonuclease
Which of the following is a mismatch? ____________
A.Polymerase – Taq polymerase
B.Template – double stranded DNA
C.Primer – oligonucleotide
D.Synthesis – 5’ to 3’ direction
微信支付 
支付宝支付