Which of the following molecules can be analyzed using a northern blot?
A.RNA
B.Carbohydrates
C.Proteins
D.DNA
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The protein bands transferred by the western blotting are previously ______________
A.Electrophoresed
B.Heated
C.Calibrated
D.Mixed
Which of the following is untrue regarding the classic yeast two-hybrid method?
A.It is used for the detection of Protein interactions
B.Method that relies on the interaction of “bait” and “prey” proteins in molecular constructs in yeast
C.DNA-binding domain and a trans-activation domain don’t necessarily interact
D.In this strategy, a two-domain transcriptional activator is employed as a helper for determining protein–protein interactions
An alternative approach to determining protein–protein interactions is to use a large-scale affinity purification technique that involves attaching fusion tags to proteins and purifying the associated protein complexes in an affinity chromatography column.
A.True
B.False
Which of the following is an incorrect statement?
A.SAGE and DNA microarrays are both high throughput techniques that determine global mRNA expression levels
B.Studies have indicated that the gene expression measurements from these methods are highly inconsistent with each other
C.SAGE does not require prior knowledge of the transcript sequence
D.DNA microarray experiments can only detect the genes spotted on the microarray
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