A1和A3型最紧密堆积结构之间有差异,高温下A1型结构比较稳定。
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具互补粘性末端的2个DNA片段间的连接可用
A. 大肠杆菌DNA连接酶或T4DNA连接酶
B. 大肠杆菌DNA连接酶
C. T4DNA连接酶
D. 两者都不能用
两平齐末端DNA片段间的连接可用
A. 大肠杆菌DNA连接酶或T4DNA连接酶
B. 大肠杆菌DNA连接酶
C. T4DNA连接酶
D. 两者都不能用
提高重组率的方法包括
A. 加大外源DNA与载体的分子比
B. 载体分子除磷
C. 连接酶过量
D. 延长连接反应时间
1. Doctors __________are the first to admit that the treatment has side effects.2. I saw the minister of education ________in the VIP lounge (贵宾厅) at the airport.3. Look, Dad, she drew this beautiful bird ______________________.4. We’ve checked the wiring and the aerial (天线) so the problem may have to do with the television ____________.5. We’ve often made that mistake ___________.6. Did you fix the computer ______________?