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Typically, there is a very small number of pluripotent adult stem cells in each tissue &, once removed from the body, their capacity to divide is limited, making generation of large quantities of stem cells difficult.
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Epithelial stem cells in the lining of the digestive tract occur in deep crypts & give rise to several cell types: absorptive cells, goblet cells, Paneth cells, & enteroendocrine cells.
Muse cells (multi-lineage differentiating stress enduring cells) are a recently discovered pluripotent stem cell type found in multiple adult tissues, including adipose, dermal fibroblasts, & bone marrow. While rare, muse cells are identifiable by their expression of SSEA-3 & general mesenchymal stem cell markers such as CD105.
Use of stem cells from amniotic fluid can overcome the ethical objections to using human embryos as a source of cells.
Skin stem cells occur in the basal layer of the epidermis & at the base of hair follicles. The epidermal stem cells give rise to keratinocytes, which migrate to the surface of the skin & form a protective layer. The follicular stem cells can give rise to both the hair follicle & to the epidermis.
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