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In general, synthetic biology will rely more on analogies to the theory and practice of other engineering disciplines, and will instead continue to build its own identity and culture.
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In developing synthetic design and control methods, the field has essentially worked its way forwards through the central dogma of molecular biology, confined in the early years to transcription-based regulatory circuits before developing DNA-based post-transcriptional and translational control systems.
Methods for post-translational regulation are still in their infancy, as a generalized system to control synthetic and endogenous proteins has not yet been reported. As synthetic systems have become larger and lesser complex, their interactions with endogenous systems have become more pronounced.
The development of cell-based therapeutic strategies in which engineered microorganisms interface with the human gut microbiota to fight infection and chronic disease is a challenge.
The pace of progress in synthetic biology will continue to accelerate as design and testing cycles rely more on the traditional molecular cloning tools that sustained the field in its early years and increasingly on DNA synthesis and high-throughput assembly methods.
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