超星 - unit 9 - 查题吧

单选题

The digestion of mRNA during RT – PCR is carried out by the enzyme ____________

A.Exonuclease
B.RNase H
C.Bal 31
D.Endonuclease

单选题

Which of the following is a mismatch? ____________

A.Polymerase – Taq polymerase
B.Template – double stranded DNA
C.Primer – oligonucleotide
D.Synthesis – 5’ to 3’ direction

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单选题

At what temperature do annealing of DNA and primer takes place? ____________

A.42˚
B.54˚
C.74˚
D.96˚

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单选题

The type of DNA amplification where the region of DNA amplified lies on either side of a known segment __________

A.RT – PCR
B.Anchored – PCR
C.Inverse – PCR
D.Nested – PCR

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单选题

Why internal secondary structures are not preferred for primers? __________

A.Internal structures are very bulky and thus elongation is not preferred
B.Because of it, primer may fold back on itself and won’t be available for template
C.Internal secondary structures require more amount of template
D.If internal structures are present, no proof reading would be observed

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单选题