超星 - unit 9 - 查题吧

单选题

The interesting stories made everyone_____.

A. excite
B. to relax
C. exciting
D. excited

单选题

A reaction mixture for PCR consists of ____________

A.heat unstable polymerase
B.primers in a limited amount
C.deoxynucleoside triphosphate (dNTPs)
D.a region complementary to the sequence to be amplified

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单选题

These are steps taken in carrying out the PCR reaction: i) Attaching of primers by cooling ii) Denaturation of strands iii) DNA synthesis iv) Heating Which is the correct order? (Mentioned from starting to ending the reaction)

A.i)-ii)-iii)-iv)
B.ii)-i)-iii)-iv)
C.iv)-iii)-ii)-i)
D.iv)-ii)-i)-iii)

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单选题

Choose the correct statement.

A. Taq polymerase is having very high processivity
B. Processivity is defined in this case as a synthesis of DNA by polymerase
C. It requires a 5’ end for the elongation to take place
D. The maximum size of the molecules which can be synthesized is 10kbp

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单选题

Which of the following activity is not present in Taq polymerase?

A. 5’-3’ polymerase
B. 5’-3’ exonuclease
C. 3’-5’ exonuclease
D. Both 5’-3’ polymerase and 5’-3’ exonuclease

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单选题

Which end of the primer should be matched properly in order to carry out the amplification?

A.5’ end
B.3’ end
C.Both of the ends should be matched properly
D.Anyone of the ends should match

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单选题

Melting temperature is given by ____________

A.4(G+C) + 2(A+T)
B.2(G+C) + 4(A+T)
C.2(A+G) + 4(C+T)
D.4(A+G) + 2(C+T)

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单选题

What will happen if the amino acid sequence is used directly for primer designing?

A.There would be certainty because the genetic code is unique for each amino acid
B.There would be uncertainty as the genetic code is degenerate and none of the amino acid is having a unique code
C.There would be uncertainty as the genetic code is degenerate but some of the amino acids such as methionine are having a unique codon
D.The amount of uncertainty or certainty is a matter of chance

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单选题

Which of the following is favored for primer design?

A.The melting temperature should be different for both the primers
B.Primers should be long in length
C.Primers should not be complementary to each other
D.Matching should be of whole primer to the template

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单选题

Reverse transcriptase produces DNA from RNA _______

A.True
B.False

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