Questions to ask before doing a biomedical project involving animals are:
A. Why are you doing it?
B. Does what you propose to do raise ethical issues?
C. Is replacement possible?
D. Is reduction possible? Is refinement possible?
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Which of the following statements about health monitoring of experimental animals are correct?
A. Health monitoring procedures became increasingly important with the extensive use and exchange of genetically modified animals between research institutions.
B. Such animals are often subclinically infected with various unwanted agents which can be detected only by comprehensive health monitoring.
C. Health monitoring can therefore be an essential prophylactic measure and helps to avoid animal losses or other complications.
D. Some of the micro-organisms that may be present in laboratory animals (e.g. lymphocytic choriomeningitis virus, Streptobacillus moniliformis) can also infect humans (zoonoses).
Which of the following statements about 'specified pathogen-free (SPF)' of experimental animals are correct?
A. The term most frequently used to describe the microbiological quality is 'specified pathogen-free (SPF)'. This term is often abused and requires explicit definition every time it is used.
B. It means that the absence of individually listed microorganisms has been demonstrated for a population by regular monitoring of a sufficient number of animals at appropriate ages by appropriate and accepted methods.
C. "SPF" animals are morphologically and physiologically normal, well suited for modeling the situation of a human population.
Due to the very low risk of introducing agents, influences of micro-organisms on the health of the animals or on the results of experiments are less likely compared to conventionally housed animals.
How do you minimizepathogenintroductionintoexperimentalanimals:
Avoidtheuseofsharpsandtakegreatcare
B. Keephandsawayfrommouth,noseandeyes
C. Wearprotectiveglovesandcoat
D. Removeglovesandwashhandsbeforeleavingtheanimalareas
Howdoyouminimizepathogenintroductionintoexperimentalanimals:
A. Use mechanical pipettes (not mouth pipettes)
B. Never eat, drink, smoke, handle contact lenses, take or apply medicines in animal areas
C. Take care to avoid creating splashes or aerosols in animal areas
D. If creating aerosols, use a safety cabinet
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