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New technologies, such as CRISPR–Cas-mediated genome editing, will enable synthetic biologists to take a less holistic engineering approach, modifying synthetic circuits and the host genome with relative ease.
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Despite the proliferation of circuit design and construction methods, there is still very little sharing of circuit constructs between groups, as few synthetic networks are developed and then never used outside the home laboratory.
With respect to protocells, current fears are based on several unknowns: consequences of an eventual interaction between these systems and natural organisms, allergenicity, pathogenicity, etc., or the possibility of protocells interfering with natural vesicles produced by bacteria.
As regards xenobiology, concerns arise from the uncertainties of the consequences of a possible interaction with natural organisms and the behavioral characteristics of these agents in the environment.
In general, synthetic biology will rely more on analogies to the theory and practice of other engineering disciplines, and will instead continue to build its own identity and culture.
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