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As regards xenobiology, concerns arise from the uncertainties of the consequences of a possible interaction with natural organisms and the behavioral characteristics of these agents in the environment.
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In general, synthetic biology will rely more on analogies to the theory and practice of other engineering disciplines, and will instead continue to build its own identity and culture.
In developing synthetic design and control methods, the field has essentially worked its way forwards through the central dogma of molecular biology, confined in the early years to transcription-based regulatory circuits before developing DNA-based post-transcriptional and translational control systems.
Methods for post-translational regulation are still in their infancy, as a generalized system to control synthetic and endogenous proteins has not yet been reported. As synthetic systems have become larger and lesser complex, their interactions with endogenous systems have become more pronounced.
The development of cell-based therapeutic strategies in which engineered microorganisms interface with the human gut microbiota to fight infection and chronic disease is a challenge.
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