题目内容

Choose the correct statement for genomic libraries. ____________

A.Genomic libraries include the representation of the whole genome of the organism
B.Sequences such as telomeres are also represented
C.Telomeres can be readily cloned
D.The larger the size of the insert of genomic DNA in recombinants, the more is the number of recombinants required to represent the genome in the library

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To avoid ligation of separate DNA fragments, which of the enzyme is used? ____________

A.phosphatase
B.kinase
C.ligase
D.endonuclease

The loop region is single stranded. It can be cleaved by using which enzyme? ____________

A.Exonuclease
B.S1 nuclease
C.RNaseH
D.DNase

Which of the following refers to the inactivation of Insertional inactivation? ___________

A.Host
B.Gene of Interest
C.Gene of the vector
D.Gene of the host

BamHI cuts the pBR322 at just one position, within the cluster of genes that code for resistance to ___________

A.Tetracycline
B.Ampicillin
C.Kanamycin
D.Gentamycin