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In _______, researchers at SUNY Stony Brook succeeded in synthesizing the 7,741 base poliovirus genome from its published sequence, producing the second synthetic pathogen genome. This took about two years of work.
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Scientists engineered yeast by increasing farnesyl pyrophosphate production and introducing the amorphadiene synthase and cytochrome P450 genes from Artemisia annua to efficiently convert simple sugars to artemisinic acid in ______.
In ______, a minimal prokaryotic genome, the chromosome of M. genitalium JCVI-1.0, was successfully chemically synthesized and assembled. In the same year, one-step assembly of 25 chemical DNA fragments into an intact M. genitalium genome was achieved in yeast by the same research group.
A pair of synthetic gene circuits that count events — a long-stated goal for circuit engineers — was reported in _______. For one of these counter circuits, recombinase-mediated DNA rearrangement was used to create permanent memory of an event, and a similar strategy was later used to engineer a full set of recombinase-based logic gates.
In ______, the first synthetic yeast genome project (Sc. 2.0 project) was launched to redesign and chemically synthesize the entire S. cerevisiae genome.
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