题目内容

If the genome size is small, then sequencing of the entire genome is ___________

A.easy
B.difficult
C.independent of the size of genome
D.not possible

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Which of the following molecules can be analyzed using a northern blot?

A.RNA
B.Carbohydrates
C.Proteins
D.DNA

The protein bands transferred by the western blotting are previously ______________

A.Electrophoresed
B.Heated
C.Calibrated
D.Mixed

Which of the following is untrue regarding the classic yeast two-hybrid method?

A.It is used for the detection of Protein interactions
B.Method that relies on the interaction of “bait” and “prey” proteins in molecular constructs in yeast
C.DNA-binding domain and a trans-activation domain don’t necessarily interact
D.In this strategy, a two-domain transcriptional activator is employed as a helper for determining protein–protein interactions

An alternative approach to determining protein–protein interactions is to use a large-scale affinity purification technique that involves attaching fusion tags to proteins and purifying the associated protein complexes in an affinity chromatography column.

A.True
B.False

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