If the genome size is small, then sequencing of the entire genome is ___________
A.easy
B.difficult
C.independent of the size of genome
D.not possible
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Which of the following molecules can be analyzed using a northern blot?
A.RNA
B.Carbohydrates
C.Proteins
D.DNA
The protein bands transferred by the western blotting are previously ______________
A.Electrophoresed
B.Heated
C.Calibrated
D.Mixed
Which of the following is untrue regarding the classic yeast two-hybrid method?
A.It is used for the detection of Protein interactions
B.Method that relies on the interaction of “bait” and “prey” proteins in molecular constructs in yeast
C.DNA-binding domain and a trans-activation domain don’t necessarily interact
D.In this strategy, a two-domain transcriptional activator is employed as a helper for determining protein–protein interactions
An alternative approach to determining protein–protein interactions is to use a large-scale affinity purification technique that involves attaching fusion tags to proteins and purifying the associated protein complexes in an affinity chromatography column.
A.True
B.False